Voltammetric Evaluation of the Binding between Wheat Germ Agglutinin and Thionine/Glucose-modified Magnetic Microbeads

SUGAWARA Kazuharu, SENBONGI Atsushi, KAMIYA Naoto, HIRABAYASHI George, KURAMITZ Hideki

doi:10.2116/analsci.24.717

Voltammetric Evaluation of the Binding between Wheat Germ Agglutinin and Thionine/Glucose-modified Magnetic Microbeads

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SUGAWARA Kazuharu

Faculty of Education, Gunma University
SENBONGI Atsushi

Faculty of Education, Gunma University
KAMIYA Naoto

Faculty of Education, Gunma University
HIRABAYASHI George

Faculty of Education, Gunma University
KURAMITZ Hideki

Department of Environmental Biology and Chemistry, Faculty of Science, University of Toyama

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The binding between glucose residues and wheat germ agglutinin (WGA) on thionine/glucose-modified magnetic microbeads was evaluated using voltammetry. Thionine is an electroactive compound and has two amino groups. Thionine was immobilized to magnetic beads via cross-linking of the amino groups on the beads with an amino group on thionine. Glucose was bound to the other amino group of thionine via the formation of a Schiff base. The beads were only several micrometers in size the same size, as cells. WGA-binding to glucose on the bead surface blankets the thionine moiety. Thus, WGA-binding could be detected as a decrease in peak current of the thionine moiety.