Quantitative Measurement of 17β-Estradiol and Estriol in River Water by Time-Resolved Fluoroimmunoassay
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- MAJIMA Keisuke
- Department of Chemistry, Waseda University, Advanced Research Institute for Science and Engineering
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- FUKUI Takashi
- Department of Chemistry, Waseda University, Advanced Research Institute for Science and Engineering
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- YUAN Jingli
- Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
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- WANG Guilan
- Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
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- MATSUMOTO Kazuko
- Department of Chemistry, Waseda University, Advanced Research Institute for Science and Engineering
書誌事項
- タイトル別名
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- Quantitative Measurement of 17.BETA.-Estradiol and Estriol in River Water by Time-Resolved Fluoroimmunoassay.
- Quantitative Measurement of 17 ベータ Estradiol and Estriol in River Water by Time Resolved Fluoroimmunoassay
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抄録
A sensitive method for detecting 17β-estradiol (E2) and estriol (E3) in river water has been developed, based on the time-resolved fluoroimmunoassay by using a fluorescent europium chelate label, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu3+. In the E2 assay, microtiter plates were coated with the E2-bovine serum albumin (BSA) conjugate. The anti-17β-estradiol antibody, the biotinylated goat anti-rabbit IgG antibody and the BHHCT-Eu3+ labeled streptavidin (SA)-BSA conjugate were used. In the E3 assay, the goat anti-rabbit IgG antibody was coated on a microtiter plate. The anti-estriol antibody and the BHHCT-Eu3+ labeled E3-BSA conjugate were used. The detection limits for E2 and E3 were 2.3 pg/ml and 4.3 pg/ml, respectively, and the analytical recoveries were 95 - 120%. Quantitative measurement of estrogens in river water was carried out for Kanda River (Tokyo, Japan) by using the method. The E2 and E3 levels were 32 pg/ml and 5.5 pg/ml, respectively. The detection limits of the present method are in the same orders of magnitude as those of ELISA for E2, and are 1 - 2 orders of magnitude better for E3.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 18 (8), 869-874, 2002
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204256873216
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- NII論文ID
- 10011311351
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD38Xmtl2jsb0%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 6261517
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- PubMed
- 12200831
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可