Purification of Pyridylaminated Oligosaccharides Using 1,2-Dichloroethane Extraction
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- SUZUKI Yusuke
- College of Science and Technology, Nihon University
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- OKANO Aya
- College of Science and Technology, Nihon University
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- KABAYAMA Kazuya
- Department of Chemistry, Graduate School of Science, Osaka University
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- NISHINA Atsuyoshi
- College of Science and Technology, Nihon University
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- TANIGAWA Minoru
- College of Science and Technology, Nihon University
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- NISHIMURA Katsushi
- College of Science and Technology, Nihon University
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- KUSHI Yasunori
- College of Science and Technology, Nihon University
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Description
Fluorescence derivatization of the oligosaccharides released from glycoconjugates is widely used for precise structural characterization. To ensure labeling of the oligosaccharides, a large excess of fluorescence reagents is usually added to the reaction tube. Therefore, any excess reagents and by-products of the labeling reaction should be removed by several column chromatographies, including using a cellulose cartridge or spin columns. However, these purification steps are often time-consuming, expensive, and laborious. In this study, we found that 1,2-dichloroethane extraction could effectively and easily purify pyridylaminated oligosaccharides with a high recovery rate.
Journal
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- Analytical Sciences
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Analytical Sciences 32 (5), 487-490, 2016
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204260163456
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- NII Article ID
- 130005150941
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 027283510
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- PubMed
- 27169645
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed