Simple Determination of L-Ascorbic Acid on TLC by Visual Detection Using Autocatalytic Reaction
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- AKASAKA Kazuaki
- Shokei Gakuin University
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The L-ascorbic acid concentration in beverages was measured after separation by silica gel thin layer chromatography (TLC) by visually determining the time in autocatalytic reaction for the L-ascorbic acid spot to turn the same yellow color of the background and disappear (the end time of the induction period) after spraying the slide with a 3,6-dihydroxyxanthane solution. There was a good linear relationship between the end time of the induction period and the concentration of L-ascorbic acid for concentrations in the range of 5.0 – 20 mM (r2 = 0.9944). In addition, there was a good relationship expressed by a quadratic equation in the concentration range of 0.1 – 5.0 mM (r2 = 0.9975). The relative standard deviations of the L-ascorbic acid values for 3 beverages (2.2 – 8.6 mM) were less than 5% (n = 5), and the recovery of 5.0 mM L-ascorbic acid from 4 beverages (0.7 – 7 mM) was 97 – 110%. A good correlation was also observed between the L-ascorbic acid values of 23 beverages (0 – 86 mM) determined by the proposed TLC method and the colorimetric method contained in a commercially available kit for L-ascorbic acid (r2 = 0.9945).
収録刊行物
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- Analytical Sciences
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Analytical Sciences 29 (5), 505-509, 2013
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204260767616
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- NII論文ID
- 10031170978
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC3snislejtw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 024473641
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- PubMed
- 23665622
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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- 使用不可