飢餓処理がパーティクルガンによりトルコギキョウ根組織に導入されたトランスジーンの一過的遺伝子発現効率を上昇させる

DOI Web Site 参考文献15件 オープンアクセス

書誌事項

タイトル別名
  • Starvation Treatment Increases Transient Expression Frequency of a Foreign Gene Introduced into Eustoma grandiflorum Root Tissues by Particle Bombardment.
  • Starvation Treatment Increases Transien

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説明

Root explants of Eustoma grandiflorum were bombarded with plasmid pBI221 harboring the uidA gene encoding β-glucuronidase (GUS) driven by a cauliflower mosaic virus 35S promoter, and the effects of pre- and post-bombardment culture in various starvation media on the number of blue spots of GUS-expressing cells were studied. It was found that pre- and post-bombardment culture in nitrogen-depleted media increased the expression frequency of the transgene, by 4- to 8-times that of the control, and that 3-h pre-culture in the nitrogen-depleted medium gave the highest expression frequency.

収録刊行物

  • 植物組織培養

    植物組織培養 13 (3), 311-315, 1996

    日本植物細胞分子生物学会

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