Functional genomics of the Dof transcription factor family genes in suspension-cultured cells of Arabidopsis thaliana

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The Dof (DNA-binding with one finger) genes are members of a family of plant-specific transcription factors that have a highly conserved DNA-binding domain, namely, Dof domain. The Dof domain is a particular class of zinc finger domain that has been demonstrated to bind specifically to DNA sequences with a T/AAAAG core. In the Arabidopsis genomic database, 36 Dof genes have been identified, whereas the functions of most of the members still remain to be studied. Therefore, we attempted to comprehensively and systematically investigate functions of Arabidopsis Dof genes. As the first step, we isolated cDNAs of all the Arabidopsis Dof genes based on the coding sequences identified on the genomic database. Then, we selected genes, which are subjected to further functional analysis, through a phylogenetic analysis, and transformed Arabidopsis cultured cells (line T87) using cDNAs corresponding to the selected genes. After that, we examined transcriptional profiles in the Dof gene-overexpressed calli using an Arabidopsis DNA microarray. From the results, possible involvements of the Dof genes in regulation of metabolic pathways are discussed.

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