Specificity of a fluorogenic DNA probe for species of a Vibrio core group.
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- SHIBA TSUNEO
- National Fisheries University
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- MURAKAMI MASATADA
- National Fisheries University
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- OHISO KAORI
- National Fisheries University
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- MATSUO YUKA
- National Fisheries University
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- MAEDA TOSHIMICHI
- National Fisheries University
書誌事項
- タイトル別名
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- Specificity of a Fluorogenic DNA Probe
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Specificity of a DNA probe developed for some species of a Vibrio core group was examined using a fluorescence resonance energy transfer system. The probe was designed based on the sequence of a 16S rDNA fragment of Vibrio parahaemolyticus cloned in a plasmid, pTPR1. The 5'-and 3'-end of the probe was ligated with fluorogenic dyes. The specificity was examined against clonal 16S rDNA fragments and chromosomal DNAs extracted from different species of Vibrio. The 16S rDNA fragments, cloned from V. parahaemolyticus, Vibrio natriegens, Vibrio fischeri, Vibrio mediterranei, and Vibrio tubiashii, had from one to four nucleotide substitutions compared with pTPR1 in the probe region. Probe hybridization with the clonal or chromosomal DNA fragments was monitored by determining the increase in fluorescence during PCR amplification. At the highest stringent condition, the probe hybridized only to identical sequences, but not to the sequences with substitution.
収録刊行物
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- Microbes and environments
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Microbes and environments 13 (3), 129-135, 1998
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204344249088
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- NII論文ID
- 110001272818
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- NII書誌ID
- AA11173196
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- ISSN
- 13474405
- 13426311
- http://id.crossref.org/issn/13426311
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- NDL書誌ID
- 4583045
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可