Dependence of Steady-State Catalytic Current on the Thickness of an Enzyme-Mediator-Immobilized Layer Fabricated by Layer-by-Layer Method

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  • ISHII Akio
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • TSUJIMURA Seiya
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • KANO Kenji
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

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Other Title
  • 酵素・メディエーター積層修飾電極における触媒定常電流の膜厚依存性
  • コウソ メディエーター セキソウ シュウショク デンキョク ニ オケル ショクバイ テイジョウ デンリュウ ノ マクアツ イソンセイ

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Description

The dependence of the bioelectrocatalytic current on the thickness of an enzyme-mediator modified layer thickness was investigated. The layer was fabricated by an electrostatic layer-by-layer assembly method. Positively charged enzyme (PQQ-dependant glucose dehydrogenase) and polymer-bounded mediator {Os complex-coordinated poly(N -vinylimidazole)} are successively entrapped on the electrode surface with negatively charged polymer (κ-carrageenan). The steady-state catalytic current value increased linearly with increasing the layer thickness, and reached the saturated value. The behavior was successfully interpreted based on the theory of the reaction layer with the enzymatic kinetic parameters, the concentrations of the enzyme and the mediator, and the diffusion coefficient of the mediator.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 56 (6), 419-424, 2007

    The Japan Society for Analytical Chemistry

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