Time-Resolved Total-Internal-Reflection Fluorescence Study on Molecular Interactions at Liquid/Liquid Interfaces

ISHIZAKA Shoji, UEDA Yuichi, NISHIJIMA Yoshiaki, KITAMURA Noboru

doi:10.2116/bunsekikagaku.54.339

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時間分解全反射蛍光法による液／液界面における分子間相互作用に関する研究
ジカンブンカイゼンハンシャケイコウホウニヨルエキエキカイメンニオケルブンシカンソウゴサヨウニカンスルケンキュウ

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Description

Molecular interactions proceeding at a water/CCl₄ interface were investigated directly by means of time-resolved total-internal-reflection (TIR) fluorescence spectroscopy. The TIR fluorescence decay profiles and fluorescence dynamic anisotropy decays of Riboflavin (RF) in the presence or absence of a N,N -dioctadecyl-[1,3,5]triazine-2,4,6-triamine (DTT) as a guest in the CCl₄ phase indicated that molecular recognition mediated by complementary triple hydrogen bonding took place effectively at the water/CCl₄ interface. Furthermore, the photoreaction mechanisms of the RF-DTT hydrogen-bonded complex that proceeded at the water/CCl₄ interface were considered based on results observed by steady state and dynamic fluorescence measurements of the RF-DTT complex under the TIR conditions. Hybridization of complementary single-stranded DNAs at a water/CCl₄ interface was also studied by the fluorescence dynamics of ethidium bromide as a probe for the DNA structure. The structures of double-stranded DNA at the interface were analyzed.<br>

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 54 (5), 339-346, 2005

The Japan Society for Analytical Chemistry

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