A Transgenic Plant that Detects Estrogenic Activity

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  • Tojo Takuto
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
  • Tsuda Kenichi
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
  • Wada Tomoko
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University
  • Yamazaki Ken-ichi
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University

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Other Title
  • 女性ホルモンを見つける植物
  • ジョセイ ホルモン オ ミツケル ショクブツ

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Abstract

A multitude of physiological processes are regulated by the endocrine system in animals. Endocrine-disrupting chemicals (EDCs) can potentially affect physiological processes of an organism by binding to steroid hormone receptors. Therefore, it is necessary to develop methods that can detect the presence of and effectively remove EDCs from the environment. We have developed an easy-to-use and inexpensive reporter gene assay system using transgenic Arabidopsis thaliana that can detect estrogenic activity. This transgenic plant constantly expresses two effector proteins, chimeric estrogen receptor and chimeric co-activator, in its nuclear. Estrogen-dependent interaction between the two effectors cause transcriptional activation of reporter gene, β-glucuronidase (GUS) . This transgenic plant has the capability to detect the existence of 17 β-estradiol at a concentration of 50 pM (13 pg/mL) in agar medium. The plant could also detect other estrogen-like substances like diethylstilbestrol, p-n-nonylphenol, bisphenol A, and genistein.

Journal

  • Waste Management Research

    Waste Management Research 15 (5), 247-253, 2004

    Japan Society of Material Cycles and Waste Management

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