Construction and Validation of Antibiotic Resistance Escherichia coli O157:H7 Strains for Acidic Foods
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- INATSU Yasuhiro
- National Food Research Institute
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- BARI Latiful
- National Food Research Institute
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- KAWASAKI Susumu
- National Food Research Institute
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- ISSHIKI Kenji
- National Food Research Institute
Bibliographic Information
- Other Title
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- 酸性食品接種試験用の抗生物質耐性病原大腸菌O157: H7株の構築と評価
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Abstract
Acid injured bacteria are sensitive to selective agents. Enumeration of such bacteria in selective media therefore results in lower estimation of viable cell counts. In this study, several antibiotic resistant Escherichia coli O157: H7 strains were constructed and their recovery in acidic conditions on several agar media was validated. Introduction of nalidixic acid or rifampicin-resistant strains did not affect either their growth rate or sensitivity against acidic challenge. Recovery of such resistant strains on tryptic-soy agar (TSA) supplemented with corresponding antibiotic was higher than that of other selective media used, and the use of antibiotic-resistant strains or TSA medium supplemented with corresponding antibiotic did not affect the estimation of recovery. These results suggested that the antibiotic resistant strains could be useful for the challenge tests of acidic food.
Journal
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- Japanese Journal of Food Microbiology
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Japanese Journal of Food Microbiology 20 (4), 177-183, 2003
Japanese Society of Food Microbiology
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Details 詳細情報について
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- CRID
- 1390001204425815808
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- NII Article ID
- 130004300492
- 80016384211
- 10012189092
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- NII Book ID
- AN10552871
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- ISSN
- 18825982
- 13408267
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- NDL BIB ID
- 6822114
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed