Influence of Water Activity on the Amount of Adenosine Triphosphate(ATP) of Bacteria by Bioluminescence.

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  • 細菌細胞中のATP量に及ぼす水分活性の影響
  • サイキン サイボウ チュウ ノ ATPリョウ ニ オヨボス スイブン カッセイ ノ エイキョウ

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ATP levels of bacteria in Tryptic Soy Broth with 0.5 to 8.7% (w/w) NaCl (water activity, aw 0.997-0.945) were studied during 3-day incubation period at 25°C by the luciferin-luciferase bioluminescence reaction method. The amount of intracellular ATP per cell of Staphylococcus epidermidis, Escherichia coli and Vibrio parahaemolyticus ranged from 1.3 to 6.1×10-18 mol/cell in the stationary phase. ATP content of bacterial cells varied greatly with age. The changes in aw had little effect upon the amount of ATP.

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