Effects of Immobilized rhBMP-2/atelocollagen in vivo and in vitro
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- Tsujigiwa Hidetsugu
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Rodriguez Andrea P.
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Takagi Tohru
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Long Hu Hai
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Rui Kan
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Lu Zhenfu
- China Medical University, Faculty of Dentistry
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- Li Xie Wei
- Harbin Medical University, Faculty of Dentistry
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- Wen-Xin Gao
- Ji Lin University, Faculty of Dentistry
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- Tan Jin
- Dalian Medical University, Faculty of Dentistry
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- Xiao Jing
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Dalian Medical University, Faculty of Dentistry
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Description
The use of recombinant human bone morphogenetic protein - 2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/atelocollagen mixture does not necessarily give adequate bone induction effect. In the present study, we evaluated the effect of immobilizing rhBMP-2 to succinylated type I atelocollagen on the cellular activity of ST2 cells and immobilized rhBMP-2/ atelocollagen and non immobilized rhBMP-2/atelocollagen implanted in subcutaneous pockets of Wister rats. Our results revealed that 1) Alkaline phosphatase activity confirmed the effectiveness of rhBMP-2/ succinylated type I atelocollagen immobilization in augmenting cellular activity. 2) Intracellular signaling continued for prolonged period when rhBMP-2 was immobilized to succinylated type I atelocollagen. 3) In rhBMP-2/atelocollagen implants were completely fully with new bone formation and cell proliferation. Whereas, in non immobilized rhBMP-2/atelocollagen implants showed new hard tissue in the periphery of the carrier with only collagen membrane in its center. This study indicated that immobilizing rhBMP-2 is an efficient method to increase bone induction.
Journal
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 15 (1), 27-33, 2006
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
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Details 詳細情報について
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- CRID
- 1390001204457670016
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- NII Article ID
- 130004480447
- 110004737681
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- NII Book ID
- AA11074332
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- COI
- 1:CAS:528:DC%2BD28XnsVOnsL8%3D
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- ISSN
- 1880828X
- 13417649
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- NDL BIB ID
- 7987614
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed