-
- Tsujigiwa Hidetsugu
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
-
- Rodriguez Andrea P.
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
-
- Takagi Tohru
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
-
- Long Hu Hai
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
-
- Rui Kan
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
-
- Lu Zhenfu
- China Medical University, Faculty of Dentistry
-
- Li Xie Wei
- Harbin Medical University, Faculty of Dentistry
-
- Wen-Xin Gao
- Ji Lin University, Faculty of Dentistry
-
- Tan Jin
- Dalian Medical University, Faculty of Dentistry
-
- Xiao Jing
- Department of Virology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Dalian Medical University, Faculty of Dentistry
この論文をさがす
説明
The use of recombinant human bone morphogenetic protein - 2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/atelocollagen mixture does not necessarily give adequate bone induction effect. In the present study, we evaluated the effect of immobilizing rhBMP-2 to succinylated type I atelocollagen on the cellular activity of ST2 cells and immobilized rhBMP-2/ atelocollagen and non immobilized rhBMP-2/atelocollagen implanted in subcutaneous pockets of Wister rats. Our results revealed that 1) Alkaline phosphatase activity confirmed the effectiveness of rhBMP-2/ succinylated type I atelocollagen immobilization in augmenting cellular activity. 2) Intracellular signaling continued for prolonged period when rhBMP-2 was immobilized to succinylated type I atelocollagen. 3) In rhBMP-2/atelocollagen implants were completely fully with new bone formation and cell proliferation. Whereas, in non immobilized rhBMP-2/atelocollagen implants showed new hard tissue in the periphery of the carrier with only collagen membrane in its center. This study indicated that immobilizing rhBMP-2 is an efficient method to increase bone induction.
収録刊行物
-
- Journal of Hard Tissue Biology
-
Journal of Hard Tissue Biology 15 (1), 27-33, 2006
硬組織再生生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204457670016
-
- NII論文ID
- 130004480447
- 110004737681
-
- NII書誌ID
- AA11074332
-
- COI
- 1:CAS:528:DC%2BD28XnsVOnsL8%3D
-
- ISSN
- 1880828X
- 13417649
-
- NDL書誌ID
- 7987614
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可