MCT118 型判定におけるバンドシフトについて

DOI Web Site Web Site 被引用文献1件 参考文献14件

書誌事項

タイトル別名
  • Electrophoretic Mobility of Amplified Products at MCT118 Locus.
  • MCT118ガタ ハンテイ ニ オケル バンドシフト ニ ツイテ

この論文をさがす

抄録

  DNA typing of MCT118 (D1S80) locus has been performed with polyacrylamide gel electrophoresis using D1S80 allelic ladder. However, some off-ladder variants, which showed different electrophoretic mobility compared with the allelic ladder, were observed frequently in MCT118 typing. Five variants from five previously typed individuals were selected for sequence analysis. The sequence of the variants were determined to ascertain whether sequence variation or size variation is the cause of altered migration of the off-ladder variants. All of the variants have nucleotide substitutions resulting in different sequences of some repeat units and do not have insertions or deletions. Consequently, the MCT118 allelic polymorphism is due to variation in the number of repeat units and to sequence variation among repeats. Furthermore, we examined electrophoresis conditions in order to accurately determine the type of MCT118. Under suitable electrophoresis conditions, all of the variants were typed as corresponding alleles within ±0.15 repeats.<br>

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (14)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ