Electrophoretic Mobility of Amplified Products at MCT118 Locus.

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  • Watanabe Gotaro
    Forensic Science Laboratory, Yamagata Prefectural Police Headquarters

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  • MCT118 型判定におけるバンドシフトについて
  • MCT118ガタ ハンテイ ニ オケル バンドシフト ニ ツイテ

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Abstract

  DNA typing of MCT118 (D1S80) locus has been performed with polyacrylamide gel electrophoresis using D1S80 allelic ladder. However, some off-ladder variants, which showed different electrophoretic mobility compared with the allelic ladder, were observed frequently in MCT118 typing. Five variants from five previously typed individuals were selected for sequence analysis. The sequence of the variants were determined to ascertain whether sequence variation or size variation is the cause of altered migration of the off-ladder variants. All of the variants have nucleotide substitutions resulting in different sequences of some repeat units and do not have insertions or deletions. Consequently, the MCT118 allelic polymorphism is due to variation in the number of repeat units and to sequence variation among repeats. Furthermore, we examined electrophoresis conditions in order to accurately determine the type of MCT118. Under suitable electrophoresis conditions, all of the variants were typed as corresponding alleles within ±0.15 repeats.<br>

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