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- 渡邉 剛太郎
- 山形県警察本部科学捜査研究所
書誌事項
- タイトル別名
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- Electrophoretic Mobility of Amplified Products at MCT118 Locus.
- MCT118ガタ ハンテイ ニ オケル バンドシフト ニ ツイテ
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抄録
DNA typing of MCT118 (D1S80) locus has been performed with polyacrylamide gel electrophoresis using D1S80 allelic ladder. However, some off-ladder variants, which showed different electrophoretic mobility compared with the allelic ladder, were observed frequently in MCT118 typing. Five variants from five previously typed individuals were selected for sequence analysis. The sequence of the variants were determined to ascertain whether sequence variation or size variation is the cause of altered migration of the off-ladder variants. All of the variants have nucleotide substitutions resulting in different sequences of some repeat units and do not have insertions or deletions. Consequently, the MCT118 allelic polymorphism is due to variation in the number of repeat units and to sequence variation among repeats. Furthermore, we examined electrophoresis conditions in order to accurately determine the type of MCT118. Under suitable electrophoresis conditions, all of the variants were typed as corresponding alleles within ±0.15 repeats.<br>
収録刊行物
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- 日本鑑識科学技術学会誌
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日本鑑識科学技術学会誌 6 (1), 43-48, 2001
日本法科学技術学会
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詳細情報 詳細情報について
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- CRID
- 1390001204484327424
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- NII論文ID
- 130004853077
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- NII書誌ID
- AA11919867
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- ISSN
- 18822827
- 13428713
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- NDL書誌ID
- 5903740
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可