Ordering and Mapping of Cosmid Clones under Semi-automated System

DOI

Bibliographic Information

Other Title
  • 自動化システムによるコスミドクローンの整列化と物理地図作成

Description

To construct the physical map of the human chromosome 21 (HC21) with ordered sets of cosmid clones (contig), we have introduced a HC21 specific arrayed library from LLNL and assembled the clones by the fingerprinting method of Carrano et. al.(1) Combination of three commercial available machines, Plasmid DNA Isolator (PI-100, Kurabo), Chemical Robot (DSP-240, Seiko) and Fluorescent Sequencer (DNA sequencer 373A, ABI) have enabled us to analyze a large number of cosmid clones. The software package (Contig Mapper 680A. ABI) has assembled the overlapping clones into contigs which will be mapped by dot blotting analysis with Alu-PCR products of HC21-hybrid cell panel. The dot scorer developed in collaboration with Hitachi SK recorded signals on high-density filters and assigned them to the arrayed library that was able to map them according to the panel. The mapped clones will provide starting materials to be sequenced.

Journal

  • Genome Informatics

    Genome Informatics 3 121-124, 1992

    Japanese Society for Bioinformatics

Details 詳細情報について

  • CRID
    1390001204490213120
  • NII Article ID
    130003997563
  • DOI
    10.11234/gi1990.3.121
  • ISSN
    2185842X
    09199454
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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