自動化システムによるコスミドクローンの整列化と物理地図作成

DOI

書誌事項

タイトル別名
  • Ordering and Mapping of Cosmid Clones under Semi-automated System

説明

To construct the physical map of the human chromosome 21 (HC21) with ordered sets of cosmid clones (contig), we have introduced a HC21 specific arrayed library from LLNL and assembled the clones by the fingerprinting method of Carrano et. al.(1) Combination of three commercial available machines, Plasmid DNA Isolator (PI-100, Kurabo), Chemical Robot (DSP-240, Seiko) and Fluorescent Sequencer (DNA sequencer 373A, ABI) have enabled us to analyze a large number of cosmid clones. The software package (Contig Mapper 680A. ABI) has assembled the overlapping clones into contigs which will be mapped by dot blotting analysis with Alu-PCR products of HC21-hybrid cell panel. The dot scorer developed in collaboration with Hitachi SK recorded signals on high-density filters and assigned them to the arrayed library that was able to map them according to the panel. The mapped clones will provide starting materials to be sequenced.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390001204490213120
  • NII論文ID
    130003997563
  • DOI
    10.11234/gi1990.3.121
  • ISSN
    2185842X
    09199454
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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