書誌事項
- タイトル別名
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- Change in DNA Bases, Deoxynucleosides and Deoxynucleotides by γ-Irradiation
- ガンマセン ショウシャ ニヨル DNA エンキ デオキシヌクレオシド オヨビ
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抄録
Aqueous solutions of DNA bases, deoxynucleosides, deoxynucleotides, and mixtures of DNA base and deoxyribose were γ-irradiated in the presence of air or in nitrogen atmosphere. Each solution was prepared at a concentration of 5×10-4M.<br> UV absorption spectra were measured directly on the test solutions, while the remaining substances and unaltered liberated bases were determined after paper chromatographic separation. The UV absorption spectra were not always depressed with decreases in remaining substances when treated with higher irradiated dose level. Adenine and cytosine appeared to be more stable in free form than in deoxynucleoside or deoxynucleotide form in nitrogen atmosphere.<br> Ammonia and inorganic phosphate were also determined. Ammonia formation was low from thymine, thymidine and thymidine monophosphate, which have no amino group in their side chains. It will be noted that nitrogen in ring scarcely contributes to ammonia formation. Inorganic phosphate formation from nucleoside of pyrimidines was greater than from that of purines.<br> It was observed by CD spectrometry that the conformation change of deoxycytidine by γ-irradiation was greater than that of deoxycytidine 5'-monophosphate.
収録刊行物
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- 日本農芸化学会誌
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日本農芸化学会誌 54 (7), 517-525, 1980
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204506921472
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- NII論文ID
- 130001227326
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- NII書誌ID
- AN00196191
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- ISSN
- 18836844
- 00021407
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- NDL書誌ID
- 2187345
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可