Measurement of Lactose in Bovine Raw Milk Using Oligosaccharide Oxidase.

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  • オリゴ糖酸化酵素を利用した生乳中ラクトースの定量
  • オリゴトウ サンカ コウソ オ リヨウ シタ セイニュウ チュウ ラクトース ノ テイリョウホウ

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Abstract

A novel method for measuring lactose in bovine raw milk has been developed. The method is based on measuring H2O2 produced from lactose by oligosaccharide oxidase through a coupled reaction involving peroxidase with a chromogenic reagent. The assay system gave a linear dose response of Lactose in the range of 25-300 μg/mL in 2-20 min of incubation. Lactose concentration in diluted bovine raw milk was directly determined with high precision by this method without any pretreatment. This is a quick and simple procedure and is suitable for multi-sample assay compared to the conventional Lane-Eynon method.

Journal

  • Nippon Nōgeikagaku Kaishi

    Nippon Nōgeikagaku Kaishi 75 (4), 447-449, 2001

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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