Spectroscopic Analysis and Electron Microscopic Observation of Lutein Dispersed in Bile Acid Solutions.

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  • TAKAGI Shigeaki
    Department of Agricultural Science, Faculty of Agriculture, Okayama University
  • ITO Takayuki
    Department of Agricultural Science, Faculty of Agriculture, Okayama University
  • NAKAJIMA Akio
    Department of Agricultural Science, Faculty of Agriculture, Okayama University
  • KIMURA Yoshinobu
    Department of Agricultural Science, Faculty of Agriculture, Okayama University
  • TAKEDA Kunio
    Department of Agricultural Science, Faculty of Agriculture, Okayama University Department of Applied Chemistry, Faculty of Technology, Okayama University of Science

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Other Title
  • 遊離型胆汁酸塩によって分散されたルテインの分光学的挙動と形態
  • ユウリガタ タンジュウサンエン ニ ヨッテ ブンサンサレタ ルテイン ノ ブン

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Description

Spectroscopic and electron microscopic studies of lutein dispersed in aqueous solutions of the bile acids, sodium cholate (CA), sodium deoxycholate (DCA), and sodium chenodeoxycholate (CDCA) were done. The bile acids caused the lutein molecules to form water-soluble aggregates of right-handed helices, which gave characteristic ellipticities in the wavelength range of 350-450 nm. The molar ellipticity of lutein increased during aggregation. The ellipticities of lutein wax maximum at about 26mM CA, 3mM DCA, and 7mM CDCA. The maximum ellipticity of lutein in CA was larger than those in the two other bile acids. This means that CA makes more regular lutein aggregate than DCA or CDCA. The binding ratio of CA to lutein was estimated to be 1000-3000mol/mol, but that of DCA to lutein was 80mol/mol. In the CA solution, large rod-shaped lutein aggregates 1.5-3μm long and 0.2-0.8μm in diameter were observed under the electron microscope. Small spherical particles 0.3μm in diameter adhered to the lutein aggregates. The number of these particles increased as the CA concentration increased, indicating that they were CA micelles. Therefore, lutein seemed to interact with the CA micelles.

Journal

  • Nippon Nōgeikagaku Kaishi

    Nippon Nōgeikagaku Kaishi 67 (7), 1055-1060, 1993

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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