PCR法によるBacillus thuringiensis cry II遺伝子の増幅と同定
書誌事項
- タイトル別名
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- Amplification and identification of cry II genes from Bacillus thuringiensis by PCR procedure.
説明
Oligonucleotide primers including a specific domain of cry II gene sequences were synthesized in order to identify cry II genes which encode the expression of the P2 protein in Bacillus thuringiensis (Bt) strains. The cry II genes of the Bt isolates were amplified by polymerase chain reaction (PCR) and the amplified DNA was used as DNA probe for identification. Cry II B was distinguished from cry II A by Hinc II digestion. The cry II genes in the Bt isolates were identified by agarose gel electrophoresis. Based on the identification of the Bt subspecies, kurstaki HD-1, aizawai IPL and galleriae harboured both cry II A and cry II B genes, which kenyae harboured only the cry II A gene. This procedure was effective for the identification of Bt isolates including both lepidopteracidal and dipteracidal proteins.
収録刊行物
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- 日本蚕糸学雑誌
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日本蚕糸学雑誌 62 (3), 223-227, 1993
社団法人 日本蚕糸学会
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詳細情報 詳細情報について
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- CRID
- 1390001204528749056
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- NII論文ID
- 130003834789
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- ISSN
- 1884796X
- 00372455
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可