ＤＮＡハイブリダイゼーション法によるＢａｃｉｌｌｕｓ　ｔｈｕｒｉｎｇｉｅｎｓｉｓの家蚕殺虫活性判定

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  • Identification of insecticidal activity of Bacillus thuringiensis against the silkworm by DNA hybridization method.
  • DNA ハイブリダイゼーションホウ ニ ヨル Bacillus thuring

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In order to identify the insecticidal activity of 20 Bacillus thuringiensis subspecies which produce bipyramidal crystals, dot blot hybridization of DNA purified from the subspecies was performed with a cloned crystal protein gene (cryl-1 gene) from subsp. kurstaki HD-1. The DNAs from the ten subspecies exhibiting on insecticidal activity against the silkworm, Bombyx mori, strongly hybridized with the probe prepared from the cloned crystal protein gene. However, DNAs from the other ten subspecies lacking insecticidal activity did not hybridize with the probe. Similar results were also obtained in the experiment using a probe prepared from the DNA fragment that is specific for insecticidal activity. Based on these results, we concluded that the intensity of the insecticidal activity against the silkworm can be determined DNA hybridization.

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