カイコ卵特異たんぱく質cDNAの大腸菌発現ベクターによるクローニング

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タイトル別名
  • Cloning of a cDNA coding for egg-specific protein of the silkworm, Bombyx mori, using an Escherichia coli expression vector.
  • カイコ卵特異タンパク質cDNAの大腸菌発現ベクターによるクローニング〔英文〕
  • カイコ ラン トクイ タンパクシツ c DNA ノ ダイチョウキン ハツゲン

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抄録

Egg-specific protein (ESP) which is identified as one of the major yolk proteins of the silkworm, Bombyx mori, is found to be synthesized only in developing ovaries. A cDNA expression library was constructed from ovarian poly (A)+ RNA and an Escherichia coli expression vector, pKEN 602, according to the vector-primer method, cDNA clones for ESP were selected by in situ colony radioimmunoassay using an anti-ESP serum. One of them, a 1.9kb cDNA insert, was identified as an essentially full-length ESP cDNA by the fact that the amino acid sequence predicted from the nucleotide sequence of 5′-terminus matched with the N-terminal amino acid sequence estimated chemically on purified ESP. Moreover, the nucleotide sequence predicts the presence of the signal peptide consisting of 18 amino acids at the N-terminus.

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