プライマーKAI01とKAI02を用いたPCRによるNosema bombycisの識別

DOI 日本農学文献記事索引 Web Site 参考文献12件
  • 川上 裕司
    Laboratory of Environmental Biology, FCG Research Institute, Inc.
  • 岩野 秀俊
    College of Bioresource Sciences, Nihon University
  • 畠山 吉則
    College of Bioresource Sciences, Nihon University Present address: Laboratory of Silkworm Pathology, Department of Sericulture, National Institute of Sericultural and Entomological Science
  • 井上 正
    College of Bioresource Sciences, Nihon University
  • CANNING ELIZABETH U.
    Imperial College at Silwood Park
  • 石原 廉

書誌事項

タイトル別名
  • Use of PCR with the specific primers for discrimination of <i>Nosema bombycis</i>
  • プライマーKAI01とKAI02を用いたPCRによる <i>Nosema bombycis</i> の識別
  • Use of PCR with the specific primers for discrimination of Nosema bombycis

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説明

The specific amplification by PCR using a pair of primers, KAI01 and KAI02 (KAWAKAMI et al., 1995) was reinvestigated as to its ability to distinguish microsporidia belonging to genus Vairimorpha, Pleistophora and Nosema. DNA samples from microsporidia of genus Vairimorpha or Pleistophora so far tested, three species of Vairimorpha and one Pleistophora, failed to produce PCR products by those primers. Of Nosema spp., N. apis, N. furnacalis, N. mylittensis and Nosema tyriae from cinnabar moth, Tyria jacobeae did not also produce any product. Microsporidian parasites isolated from silkworms infected with pebrine in the northern part of India (courtesy of Dr. A. K. Bansal) and in southern India (courtesy of Dr. KIYOSHI KAWAKAMI) and in China (courtesy of Dr. X. CHEN) gave PCR products by KAI01 and KAI02 primers. The results from our study suggest that PCR tests with the use of KAI01 and KAI02 primers are useful to distinguish highly virulent strains of N. bombycis in pebrine inspection.

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