Bioluminescent Reaction by Immobilized Luciferase

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  • Tanaka Ryuta
    Department of quantum matter, ADSM, Hiroshima University
  • Takahama Eriko
    Department of quantum matter, ADSM, Hiroshima University
  • Iinuma Masataka
    Department of quantum matter, ADSM, Hiroshima University
  • Ikeda Takeshi
    Research Institute for Nanodevice and Bio Systems, Hiroshima University
  • Kadoya Yutaka
    Department of quantum matter, ADSM, Hiroshima University
  • Kuroda Akio
    Department of molecular biotechnology, ADSM, Hiroshima University

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Other Title
  • 固定したルシフェラーゼによる生物発光反応
  • コテイ シタ ルシフェラーゼ ニ ヨル セイブツ ハッコウ ハンノウ

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We have investigated an effect of immobilization of luciferase molecules at the optical fiber end on a bioluminescent reaction. The time dependence of measured count rates of emitted photons has been analyzed by fitting with numerical solution of differential equations including the effect of the product-inhibitor and the deactivation of the luciferase. Through the analysis, we have successfully extracted kinetic constants such as, reaction rate, number of active luciferase molecules, etc. Ratio of active molecules to total luciferase molecules in immobilization was one order of magnitude lower than that in solution. The reaction rate of the bioluminescent process was also different from the one of free luciferase in solution.

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