書誌事項
- タイトル別名
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- Bioluminescent Reaction by Immobilized Luciferase
- コテイ シタ ルシフェラーゼ ニ ヨル セイブツ ハッコウ ハンノウ
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説明
We have investigated an effect of immobilization of luciferase molecules at the optical fiber end on a bioluminescent reaction. The time dependence of measured count rates of emitted photons has been analyzed by fitting with numerical solution of differential equations including the effect of the product-inhibitor and the deactivation of the luciferase. Through the analysis, we have successfully extracted kinetic constants such as, reaction rate, number of active luciferase molecules, etc. Ratio of active molecules to total luciferase molecules in immobilization was one order of magnitude lower than that in solution. The reaction rate of the bioluminescent process was also different from the one of free luciferase in solution.
収録刊行物
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- 電気学会論文誌C(電子・情報・システム部門誌)
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電気学会論文誌C(電子・情報・システム部門誌) 131 (1), 23-28, 2011
一般社団法人 電気学会
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詳細情報 詳細情報について
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- CRID
- 1390001204607125504
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- NII論文ID
- 10027636556
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- NII書誌ID
- AN10065950
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- ISSN
- 13488155
- 03854221
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- NDL書誌ID
- 10929276
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDLサーチ
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- 使用不可