Decline in Glucokinase Activity in the Arcuate Nucleus of Streptozotocin-Induced Diabetic Rats
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- Nishio Takashi
- Laboratory of Neuropsychopharmacology, Graduate School of Environmental and Human Sciences, Meijo University
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- Toyoda Yukiyasu
- Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University
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- Hiramatsu Masayuki
- Laboratory of Neuropsychopharmacology, Graduate School of Environmental and Human Sciences, Meijo University
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- Chiba Taku
- Faculty of Pharmacy, Kinjo Gakuin University
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- Miwa Ichitomo
- Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University
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Description
Glucokinase (GK) is known to be the critical glucose sensor of pancreatic B-cells. However, the localization and functional role of GK in the brain remains to be elucidated. In this study, we measured both the activity and mRNA level of GK in the hypothalamic nuclei and the cortex of rats injected intraperitoneally with streptozotocin or vehicle. GK activity was measured by a fluorometric assay; and the GK mRNA level, by use of the real-time reverse transcription polymerase chain reaction. GK activity in vehicle-treated rats was high in the arcuate nucleus, moderate or low in the ventromedial nucleus, lateral hypothalamic area, and paraventricular nucleus, and very low in the cortex. The order of GK mRNA level was almost the same as that of GK activity. GK activity and GK mRNA level only in the arcuate nucleus of streptozotocin-treated rats at 7 d, but not at 2 d, after treatment were lower than those of vehicle-treated rats. The results suggest that prolonged hyperglycemia induced by diabetes decreased the activity of GK in the arcuate nucleus.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 29 (2), 216-219, 2006
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204624700928
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- NII Article ID
- 110005663948
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 7791479
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed