Decline in Glucokinase Activity in the Arcuate Nucleus of Streptozotocin-Induced Diabetic Rats

DOI Web Site Web Site 被引用文献1件 参考文献44件
  • Nishio Takashi
    Laboratory of Neuropsychopharmacology, Graduate School of Environmental and Human Sciences, Meijo University
  • Toyoda Yukiyasu
    Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University
  • Hiramatsu Masayuki
    Laboratory of Neuropsychopharmacology, Graduate School of Environmental and Human Sciences, Meijo University
  • Chiba Taku
    Faculty of Pharmacy, Kinjo Gakuin University
  • Miwa Ichitomo
    Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University

この論文をさがす

説明

Glucokinase (GK) is known to be the critical glucose sensor of pancreatic B-cells. However, the localization and functional role of GK in the brain remains to be elucidated. In this study, we measured both the activity and mRNA level of GK in the hypothalamic nuclei and the cortex of rats injected intraperitoneally with streptozotocin or vehicle. GK activity was measured by a fluorometric assay; and the GK mRNA level, by use of the real-time reverse transcription polymerase chain reaction. GK activity in vehicle-treated rats was high in the arcuate nucleus, moderate or low in the ventromedial nucleus, lateral hypothalamic area, and paraventricular nucleus, and very low in the cortex. The order of GK mRNA level was almost the same as that of GK activity. GK activity and GK mRNA level only in the arcuate nucleus of streptozotocin-treated rats at 7 d, but not at 2 d, after treatment were lower than those of vehicle-treated rats. The results suggest that prolonged hyperglycemia induced by diabetes decreased the activity of GK in the arcuate nucleus.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (44)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ