Microdissection and micromanipulation in the scanning electron microscope.

Wakita Minoru, Shioi Takashi, Nodasaka Yoshinobu

doi:10.2330/joralbiosci1965.26.502

Microdissection under SEM was attempted by using a micromanipulator functioning with screws and equipped with a φ 0.1mm tungsten wire as the tip instrument. The specimens in these experiments, were rat embryos immersed in a 1.25% glutaraldehyde (GA)-2% paraformaldehyde (PFA) mixture and some organs from adult rats perfused with 2.5% GA-4% PFA mixture. It was shown that the Bowman's capsule of the kidney corpuscle exposed on the freely fractured surface can be removed by a tungsten probe on the micromanipulator, and to make the gromerulus visible, the pericardium of the rat embryo could be peeled off and the anlage of the heart exposed and dissected further to display the intracardial space. Microdissection of the eye of an embryo was also tried.<BR>It showed the possiblility of freely and arbitrarily making objects visible, objects which until now have been obtained only by chance. This method will have wider applications especially in embryological studies, which depend on the skill of experienced investigators.<BR>The various problems to develop more accurate microdissections were pointed out and discussed. The solutions to some of these problems are suggested.

Microdissection and micromanipulation in the scanning electron microscope.

抄録

収録刊行物

キーワード

詳細情報詳細情報について

書き出し

問題の指摘

Microdissection and micromanipulation in the scanning electron microscope.

抄録

収録刊行物

キーワード

詳細情報 詳細情報について

書き出し

問題の指摘

詳細情報詳細情報について