Changes with age in the auto-fluoresence intensity of human dentin, in the buccolingual and longitudinal directions.

  • Matsumoto Hayashi
    First Department of Oral Anatomy, School of Dentistry, Tokushima University
  • Araki Tsutomu
    Department of Mechanical Engineering, Faculty of Engineering, Tokushima University
  • Hirota Katsuhiko
    Department of Oral Microbiology and Immunology, School of Dentisby, Tokushima University
  • Kawata Terushige
    Department of Orthodontics, School of Dentistry, Tokushima University
  • Kitamur Seiichiro
    First Department of Oral Anatomy, School of Dentistry, Tokushima University
  • Yamashita Kikuji
    First Department of Oral Anatomy, School of Dentistry, Tokushima University

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Other Title
  • 頬舌および歯軸方向におけるヒト象牙質の自己蛍光強度の加齢変化

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Changes with age in the auto-fluorescence intensity of six regions in human dentin were studied in the buccolingual and longitudinal directions using a time-resolved fluorescence microphotometer. Static measurement showed that fluorescence intensity increased with age in all six regions. However, the wavelength at maximum fluorescence or at full width of half maximum in the fluorescence emission spectrum, did not change ; the former and latter values were about 440 nm and 100 nm, respectively, in all six regions of all the teeth studied. Dynamic measurement suggested that auto-fluorescence at a wavelength of 440 nm had at least three components with different fluorescence decay times ; fast, intermediate and slow.<BR>The fluorescence intensities of these components increased with age in all six regions ; the intensity of the fast component increased in proportion to age after 18 years, while the rate of the increase in the fluorescence intensities of the other two components decreased with age. Changes with age in the ratio of the intensity of the three components to the static fluorescence intensities were distinguishable longitudinally; the ratio of the fast component increased with age in coronal dentin, while the ratio of the slow component decreased with age in dentin of the root apex. Cervical dentin showed a tendency intermediate between these two regions. These results indicate that patterns of changes with age differed in the longitudinal direction, which suggests that factors that affect age changes differ between coronal and root apex dentin.

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