細胞診におけるＨＰＶ　ＤＮＡ検出のためのＩｎ　ｓｉｔｕ　ｈｙｂｒｉｄｉｚａｔｉｏｎ（ＩＳＨ）法　固定法，処理法の検討

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  • The in situ hybridization (ISH) method for detecting HPV in cytodiagnosis. A study of fixation and processing methods.
  • A study of fixation and processing methods
  • 固定法, 処理法の検討

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Using the ISH method with the objective of detecting HPV DNA in cytodiagnosis of the uterine cervix, we studied methods fixation and processing (proteopeptic response and their combinations) and obtained the following results.<BR>1) When ISH operational techniques were used in normal tissue specimens, staining was better with 15% neutral buffered formalin fixation than 95% ethanol, acetone fixation.<BR>2) When 95% ethanol fixation was used, it was possible to omit the proteopeptic reaction included in the ISH operational techniques, and favorable staining results were obtained. This indicates that, during normal cytodiagnosis it is not necessary to prepare a special fixation solution.<BR>3) From Papanicolaou staining with restaining specimens, HPV-DNA positive results were obtained. Given the above results, it is comsidered possible to conduct retrospective studies of HPV from Papanicolaou staining with restaining specimens in cytodiagnosis of the uterine cervix.

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