Cloning System for Streptomyces kasugaensis Using the Hybrid Melanin-Synthesizing Gene melE as a Chromogenic Marker
-
- Kojima Ikuo
- Department of Biotechnology, Akita Prefectural University
-
- Kasuga Kano
- Department of Biotechnology, Akita Prefectural University
-
- Kobayashi Masayuki
- Department of Biotechnology, Akita Prefectural University
-
- Matsuo Masanao
- Diagnostics Research Laboratories, Daiichi Pure Chemicals Co., Ltd.
-
- Akagawa Hisayoshi
- Department of Bioactive Molecules, National Institute of Infectious Diseases
-
- Mizuno Satoshi
- Department of Bioactive Molecules, National Institute of Infectious Diseases
この論文をさがす
説明
Streptomyces kasugaensis, a producer of kasugamycin, is listed as a particularly safe Streptomyces host in the Japan Guidelines for Recombinant DNA Experiments. In search of a chromogenic selectable marker usable for S. kasugaensis, melanin-synthesizing melE was constructed by placement of the operon for the melanin-synthesizing gene (melC) from Streptomyces antibioticus under the control of the promoter of the erythromycin-resistance gene (ermE) from Saccharopolyspora erythraea. Combined use of melE and the thiostrepton-resistance gene from Streptomyces azureus yielded vectors pSK216 and pSK221, derivatives of pSK2 from S. kasugaensis MB273. In S. kasugaensis and Streptomyces lividans, melE-harboring pSK216 and pSK221 induced detectable amounts of melanin pigment on agar media in marked contrast to pSK2-based pSK212 carrying melC, thereby establishing an efficient cloning system for S. kasugaensis.
収録刊行物
-
- 日本放線菌学会誌
-
日本放線菌学会誌 17 (2), 29-32, 2003
日本放線菌学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204701345792
-
- NII論文ID
- 130004487528
- 10012501124
-
- NII書誌ID
- AN10080091
-
- COI
- 1:CAS:528:DC%2BD2cXktVOku7s%3D
-
- ISSN
- 18816371
- 09145818
-
- NDL書誌ID
- 6876626
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可