Rapid Discrimination of Porphyra yezoensis and P. tenera by PCR-SSCP Using Restriction Fragments and Silver Stain Detection.

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  • KAMINISHI Yoshio
    Department of Food Sience and Technology, National Fisheries University
  • KUNIMOTO Masahiko
    Department of Food Sience and Technology, National Fisheries University
  • YAMAMOTO Yuji
    Department of Applied Aquabiology, National Fisheries University
  • MIZUKAMI Yuzuru
    Department of Applied Aquabiology, National Fisheries University
  • KITO Hitoshi
    Department of Applied Aquabiology, National Fisheries University

Bibliographic Information

Other Title
  • 銀染色と制限酵素断片を用いたPCR-SSCP法によるスサビノリとアサクサノリの迅速判定
  • Rapid Discrimination of Porphyra yesoensis and P. tenera by PCR-SSCP Using Restriction Fragments and Silver Stain Detection

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Description

Porphyra yezoensisandP. tenera (wild types) were discriminated by the PCR-SSCP (single strand conformation polymorphism) technique on 2 regions amplified from small-subunit ribosomal RNA genes. A simple method has been developed using silver stain detection and 5 or 6% acrylamide gel with 2.0% crosslink monomer and 10% glycerol under the buffer system of Tris-Glycine. In the 586 nucleotides region, which had more sequence variations amongPorphyrasp., single-point mutations were found using SSCP analysis of restriction fragments. PCR-SSCP analysis of 13 layer cultivars indicated the same pattern as forP. yezoensis. The modified PCR-SSCP analysis contains 4 steps and can be completed within 8 h.

Journal

  • Aquaculture Science

    Aquaculture Science 47 (1), 1-7, 1999

    Japanese Society for Aquaculture Science

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