銀染色と制限酵素断片を用いたPCR-SSCP法によるスサビノリとアサクサノリの迅速判定

DOI 日本農学文献記事索引 Web Site 参考文献20件
  • 上西 由翁
    Department of Food Sience and Technology, National Fisheries University
  • 国本 正彦
    Department of Food Sience and Technology, National Fisheries University
  • 山本 有司
    Department of Applied Aquabiology, National Fisheries University
  • 水上 譲
    Department of Applied Aquabiology, National Fisheries University
  • 鬼頭 鈞
    Department of Applied Aquabiology, National Fisheries University

書誌事項

タイトル別名
  • Rapid Discrimination of Porphyra yezoensis and P. tenera by PCR-SSCP Using Restriction Fragments and Silver Stain Detection.
  • Rapid Discrimination of Porphyra yesoensis and P. tenera by PCR-SSCP Using Restriction Fragments and Silver Stain Detection

この論文をさがす

説明

Porphyra yezoensisandP. tenera (wild types) were discriminated by the PCR-SSCP (single strand conformation polymorphism) technique on 2 regions amplified from small-subunit ribosomal RNA genes. A simple method has been developed using silver stain detection and 5 or 6% acrylamide gel with 2.0% crosslink monomer and 10% glycerol under the buffer system of Tris-Glycine. In the 586 nucleotides region, which had more sequence variations amongPorphyrasp., single-point mutations were found using SSCP analysis of restriction fragments. PCR-SSCP analysis of 13 layer cultivars indicated the same pattern as forP. yezoensis. The modified PCR-SSCP analysis contains 4 steps and can be completed within 8 h.

収録刊行物

  • 水産増殖

    水産増殖 47 (1), 1-7, 1999

    日本水産増殖学会

参考文献 (20)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ