Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L

DOI Web Site Web Site 参考文献23件
  • Huang Zhen
    State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University
  • Liu Lu
    State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University
  • Lu Hong
    State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University
  • Lang Lina
    State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University
  • Zhao Na
    State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University
  • Ding Juan
    State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University
  • Xu Aixia
    State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University

書誌事項

タイトル別名
  • Development of IP and SCAR markers linked to the yellow seed color gene in <i>Brassica juncea</i> L.
公開日
2016
DOI
  • 10.1270/jsbbs.66.175
公開者
日本育種学会

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説明

Previous studies showed that the yellow seed color gene of a yellow mustard was located on the A09 chromosome. In this study, the sequences of the molecular markers linked to the yellow seed color gene were analyzed, the gene was primarily mapped to an interval of 23.304 to 29.402M. Twenty genes and eight markers’ sequences in this region were selected to design the IP and SCAR primers. These primers were used to screen a BC8S1 population consisting of 1256 individuals. As a result, five IP and five SCAR markers were successfully developed. IP4 and Y1 were located on either side of the yellow seed color gene at a distance of 0.1 and 0.3 cM, respectively. IP1, IP2 and IP3 derived from Bra036827, Bra036828, Bra036829 separately, co-segregated with the target gene. BLAST analysis indicated that the sequences of newly developed markers showed good collinearity with those of the A09 chromosome, and that the target gene might exist between 27.079 and 27.616M. In light of annotations of the genes in this region, only Bra036828 is associated with flavonoid biosynthesis. This gene has high similarity with the TRANSPARENT TESTA6 gene, Bra036828 was hence identified as being the gene possibly responsible for yellow seed color, in our research.

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