Preparation of Novel Organosilica Nanoparticles and Their Application for Photodynamic Therapy

DOI 18 References
  • Nakamura Michihiro
    Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • Ishimura Kazunori
    Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School

Bibliographic Information

Other Title
  • 新規な有機シリカナノ粒子の作製と光線力学的治療への応用
Published
2009
Resource Type
journal article
DOI
  • 10.2530/jslsm.30.394
Publisher
Japan Society for Laser Surgery and Medicine

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Description

Nanoparticles have been proposed as potential carriers for photosensitizers. Novel silica nanoparticles made of organosilica (Organo-NPs) have been successfully synthesized by using a St?ber method. We compared the Organo-NPs to classical silica nanoparticles made of tetraethoxysilane (TEOS NPs). The Organo-NPs were well dispersed in solution and have unique surface properties such as thiol residues on the surface and reduced zeta potential compared with TEOS NPs. The Organo-NPs with fluorescent dye distributed throughout the particle were synthesized by a method that deposited fluorescent dye on the silica network via a succinimidyl ester reaction with (3-aminopropyl) trimethoxysilane and via a maleimide reaction with organosilica. The resulting Organo-NPs with fluorescent were bright, non-aggregated in solution, and photostable. The fluorescence intensity and photostability of fluorescent Organo-NPs were sufficient for detection as a single fluorescent particle using flow cytometry and fluorescence microscopy. Protein-modified Organo-NPs were easily prepared by absorption and by maleimide coupling. The Organo-NPs did not exhibit death or cytotoxic findings on in vivo injection. The Organo-NPs containing photosensitizers showed phototoxic activity in culture cells. We demonstrate the usefulness of the Organo-NPs and their surface properties, and discuss their advantages for biological applications and possibility for photodynamic therapy.

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