Evaluation of toxicities of 7-hydroxyisotrichodermin and 8-hydroxyisotrichodermin, shunt intermediates in the biosynthetic grid of deoxynivalenol, by using a sensitive yeast assay

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  • Kamata Kentaro
    Graduate School of Engineering, Toyo University
  • Tanaka Akira
    Graduate School of Engineering, Toyo University
  • Maeda Kazuyuki
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • Takushima Ryo
    Graduate School of Engineering, Toyo University
  • Sato Hiroki
    Department of Science and Engineering, Toyo University
  • Aikawa Shunichi
    Research Institute of Industrial Technology, Toyo University
  • Yoshida Yasuhiko
    Graduate School of Engineering, Toyo University Department of Science and Engineering, Toyo University Research Institute of Industrial Technology, Toyo University Bio-Nano Electronics Research Centre, Toyo University
  • Kimura Makoto
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • Takahashi-Ando Naoko
    Graduate School of Engineering, Toyo University Department of Science and Engineering, Toyo University Research Institute of Industrial Technology, Toyo University Bio-Nano Electronics Research Centre, Toyo University

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Abstract

Fusarium graminearum causes a disease of wheat and barley known as Fusarium head blight. It contaminates the grains with trichothecene mycotoxins such as deoxynivalenol (DON). As shunt intermediates in the DON biosynthetic pathway, 7-hydroxyisotrichodermin (7-HIT) and 8-hydroxyisotrichodermin (8-HIT) are known. However, their activities have not been previously evaluated. In this study, we performed toxicity assays of these trichothecenes by using a sensitive yeast bioassay that we have recently established. The IC50 of 7-HIT and 8-HIT were in the range of 20-40 μg/ml, while the IC50 of DON was approximately 1.5 μg/ml. Although the toxicity of these shunt metabolites remains to be investigated in animal systems, our present data indicate that 7-HIT and 8-HIT may not be major issues that require regulation in agricultural products.

Journal

  • JSM Mycotoxins

    JSM Mycotoxins 65 (1), 7-9, 2015

    Japanese Society of Mycotoxicology

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