二価鉄誘導脂質過酸化反応に関する研究

橋本 啓二

doi:10.4044/joma1947.94.3-4_349

二価鉄誘導脂質過酸化反応に関する研究

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橋本啓二

岡山大学医学部放射線医学教室

書誌事項

タイトル別名

Studies on ferrous ion-induced lipid peroxidation
二価鉄誘導脂質過酸化反応に関する研究-1-二価鉄誘導脂質過酸化反応液中の二価鉄の定量
2カテツユウドウシシツカサンカハンノウニカンスルケンキュウ 1 2
第一編二価鉄誘導脂質過酸化反応溶液中の二価鉄の定量
Part I Quantitative analysis of ferrous ion in a system of ferrous ion-induced lipid peroxidation

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説明

The 2-nitroso-(N-propyl-N-sulfopropylamino)phenol(Nitroso-PSAP), one of the watersoluble derivatives of nitrosoaminophenol, is a very sensitive reagent for the spectrophotometric determination of Fe (II). Using Nitroso-PSAP reagent, the author investigated the conditions for determining Fe (II) quantitatively and selectively the system of Fe (II)-induced lipid peroxidation, and obtained the following results.<br>1) Fe (II) could be measured quantitatively without reductant. The Fe (II) complex had a molar absorptivity of 4.5×10⁴ dm³ mol^-1 cm^-1 at 756 nm.<br>2) Fe (II) could be measured quantitatively in the TCA solution removed from the reaction medium for lipid peroxidation. The Fe (II) complex had a molar absorptivity of 3.14×10⁴ dm³ mol^-1 at 756 nm.<br>3) Fe (II) could be measured quantitatively and selectivily under conditions involving a mixture of Fe (II).