書誌事項
- タイトル別名
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- Insulin Binding to Plasma Membranes in Ascites Hepatoma Cells
- フクスイ カンガン サイボウ ノ インスリン レセプター ニカンスルケンキュウ
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The nature of insulin receptors in ascites hepatoma AH-66 were studied. The hepatoma plasma membranes were found to contain at least two types of insulin receptors with affinity constants of 9.0 × 109M-1 and 2.8 × 109M-1. Their affinity constants were almost equal to those in plasma membranes from normal rat liver. The capacity of the high affinity binding sites in AH-66 cells was larger by 50% as compared with that of normal liver. No differences were detected between AH-66 cells and normal livers in the inhibiting effect of concanavalin A on 125I-insulin binbing. Furthermore, neither glucagon, ACTH, TSH, nor HGH inhibited 125I-insulin binding to plasma membranes from AH-66 cells. Addition of 2mM N-ethylmaleimide to the assay system increased the affinity constant, binding capacity, and specific 125I-insulin binding.<BR>These results indicate that the plasma membrane of ascites hepatoma AH-66 has high and low affinity insulin receptors which are specific for insulin as that of the normal liver cell.
収録刊行物
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- 糖尿病
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糖尿病 20 (6), 742-747, 1977
一般社団法人 日本糖尿病学会
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詳細情報 詳細情報について
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- CRID
- 1390001204903805056
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- NII論文ID
- 130004336638
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- NII書誌ID
- AN00166576
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- ISSN
- 1881588X
- 0021437X
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- NDL書誌ID
- 1919222
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可