Ｇｌｕｃｏｓｙｌａｔｅｄ　Ａｌｂｕｍｉｎ測定法とその臨床的意義

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タイトル別名
  • Determination of Glucosylated Albumin and Its Clinical Significance in Diabetes Mellitus
  • Glucosylated Albumin ソクテイホウ ト ソノ リンショウテ

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In the present study, we report the characterization of a colorimetric assay procedure for glucosylated albumin (G-A). Moreover, the level of G-A was determined in the serum of normal and diabetic subjects after purification of the albumin to apparent homogeneity.<BR>Across the range of dilutions (2.5-15 mg/m/) of the same albumin, 5-hydroxymethylfurfural (HMF) formation as determined by the thiobarbituric acid (TBA) method was a linear function of albumin concentration. Also, HMF formation from G-A during hydrolysis at 100°C with oxalic acid was progressive in the range from 1 to 4.5 hr, and then decreased slightly at 6 hr. There was a significant correlation (r=0.84) between the G-A and HbAI levels in 43 normal and 167 diabetic subjects. The correlations between the G-A and mean fasting blood sugar (FBS) levels were studied. There was a higher correlation (r=0.67) between G-A and the mean FBS within 2 weeks. On starting insulin therapy in 8 untreate juvenile diabetic subjects, there was a different temporal relationship between the FBS, G-A and HbAI levels. The G-A levels were significantly decreased at 1, 2, 3 and 4 weeks compared to the HbAI levels.<BR>The present results indicate that G-A may provide a valuable tool for assessing the mean blood sugar levels between shorter intervals, since the turnover of serum albumin is considerably faster than that of HbAI.

収録刊行物

  • 糖尿病

    糖尿病 25 (6), 691-696, 1982

    一般社団法人 日本糖尿病学会

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