高分子小腸型ALPがアガロースゲル電気泳動法における骨型ALP分画値に及ぼす影響

DOI 被引用文献2件
  • 星野 忠
    日本大学医学部臨床検査医学系臨床検査医学分野
  • 矢内 充
    日本大学医学部臨床検査医学系臨床検査医学分野
  • 土屋 達行
    日本大学医学部臨床検査医学系臨床検査医学分野
  • 熊坂 一成
    日本大学医学部臨床検査医学系臨床検査医学分野
  • 菰田 二一
    埼玉医科大学医学部生化学

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タイトル別名
  • High-molecular-mass intestinal alkaline phosphatase affects the fraction amount of bone alkaline phosphatase measured by agarose gel electrophoresis

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We demonstrated the serum sample treated with neuraminidase and protease by agarose gel electrophoresis, and calculated the activity of bone-type alkaline phosphatase (BAP) fraction. From the result, high-molecular-mass intestinal alkaline phosphatase (HIAP) takes place to overlapping with the activity of BAP fraction. The protease-treated and non-treated samples were both subjected to agarose gel electrophoresis. Data showed a decrease in BAP in the α2β region and increase in normal-molecular-mass intestinal alkaline phosphatase in the β region were as HIAP positive. Therefore, the serum sample used for the isozyme measurement on the agalose gel electrophoresis, the protease treatment may be essential. Resulting that a good correlation (r=0.982) was found between the BAP activity determined by the mentioned-above electrophoresis method using a protease treatment, and the BAP activity determined by the ELISA method.

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