A High Accuracy and Simple Analytical Method for Microcystins Using <sup>15</sup>N－labeled Microcystins

SANO TOMOHARU, TANAKA YOSHITO, TOBIISHI KAZUHIRO, ARAHORI YASUSHI, SHIMIZU AKIRA, SATO CHIZUKO, KAYA KUNIMITSU

doi:10.2521/jswtb.52.31

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<sup>15</sup>N標識ミクロシスチンを用いた簡便で高精度なミクロシスチン分析手法
A High Accuracy and Simple Analytical Method for Microcystins Using ¹⁵N-labeled Microcystins

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Microcystins, cyclic heptapeptide hepatotoxins, are the most frequently detected from freshwater cyanobacteria around the world. WHO recommended a guideline value of microcystin－LR that the dosage should be below 1 μg・L－¹ in drinking water. To monitor microcystins concentration in water, LC－MS/MS has been used. However, ionization efficiencies of internal standards are different from those of microcystins. As the results, the accuracy of microcystin determination is not enough for the monitoring.To improve the accuracy, we developed a simple and precise method using stable isotope (¹⁵N)－labeled microcystin. As procedures, a frozen water-sample (0.5 mL) was thawed and added ¹⁵N－labeled microcystin with 0.5 mL of MeOH. After ultra-sonication for 10 min of the treated sample, the sample solution was filtered using 0.22 μm membrane filter. The filtrate was injected to LC－MS/MS and quantified. The limit of quantification was around 0.1 μg・L－¹ depended on the instrument sensitivity. Using this method, high accurate quantified results of microcystins in water sample were obtained.

Journal

Japanese Journal of Water Treatment Biology

Japanese Journal of Water Treatment Biology 52 (1), 31-34, 2016

Japanese Society of Water Treatment Biology

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