Studies of Platelet Aggregation in Six Cases of EDTA-dependent Pseudothrombocytopenia

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  • SHIMASAKI Akemi
    Department of Internal Medicine, Himeji National Hospital
  • KATO Tadahiko
    Department of Internal Medicine, Himeji National Hospital
  • OZAKI Yukio
    Department of Clinical and Laboratory Medicine, Yamanashi Medical College

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Other Title
  • EDTA依存性偽性血小板減少症6例における凝集の検討
  • EDTA イゾンセイ ギセイ ケッショウバン ゲンショウショウ 6レイ ニ オ

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Peripheral blood count was performed by a Coulter Model S Plus STKR on six pseudothrombocytopenia patients (age: 16∼70, 2 men and 4 women) using three different anticoagulants. Treatment with ethylene diamine tetraacetate (EDTA, 1 mg/ml) or sodium heparin (25 U/ml) aggregated platelets, but sodium citrate (3.8%, 1:9) had no effect. Smear examination revealed much platelet clumping but the satellite phenomenon was not present. No specific pattern was elucidated concerning cell size distribution curves between treatment by EDTA and heparin. Theophylline (10 mg/ml) and prostaglandin I2 (1 μM) inhibited EDTA-induced platelet aggregation but aspirin (1.8 mM) did not. On the other hand, these three substances inhibited heparin-induced platelet aggregation. These findings, taken together, suggested that EDTA and heparin initiated platelet activation and EDTA-induced platelet aggregation might be a process unrelated to thromboxan A2 production. Heparin may not be a suitable anticoagulant since it aggregates platelets of some healthy individuals.

Journal

  • Rinsho Ketsueki

    Rinsho Ketsueki 35 (6), 529-534, 1994

    The Japanese Society of Hematology

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