書誌事項
- タイトル別名
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- The Measurement of β-Thromboglobulin by Enzyme Immunoassay Technique
- ベータ トロンボ グロブリン ノ エンザイム イムノアッセイ ニヨル ソクテイ
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抄録
Sensitive enzyme immunoassay system for measurement of β-thromboglobulin (β-TG) in plasma was prepared with purified antibody to β-TG and β-D-galactosidase from Escherichia coli as labeled substance. The antibodies were purified with an immunoaffinity chromatography form the antiserum, which had been produced in rabbits by injecting purified β-TG. The assay system consisted of the polystyrene bead with immobilized antibody F (ab')2 fragments and the antibody Fab' fragments labeled with β-D-galactosidase. The assay was highly sensitive with β-TG measurable at levels as low as 10 pg/assay tube. β-TG levels in plasma was measured with 2.5 μl samples. Under the conditions, coefficient of variation for within and between run was less than 8% and the results obtained with the enzyme immunoassay correlated well with those with a radioimmunoassay. The plasma β-TG levels in healthy subjects determined with the present assay system were 38.4±6.2 ng/ml and the levels were markedly high in patients with myeloproliferative syndrome and in patients with DIC.
収録刊行物
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- 臨床血液
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臨床血液 23 (2), 150-154, 1982
一般社団法人 日本血液学会
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詳細情報 詳細情報について
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- CRID
- 1390001205032145280
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- NII論文ID
- 130004917970
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- NII書誌ID
- AN00252940
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- COI
- 1:STN:280:Bi2B2c3msFM%3D
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- ISSN
- 18820824
- 04851439
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- NDL書誌ID
- 2483866
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- PubMed
- 6178857
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可