Evidence for the Essential Role of Myosin Subfragment-2 in the ATP-Dependent Actin-Myosin Sliding in Muscle Contraction.
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- TSUCHIYA Teizo
- Department of Biology, Faculty of Science, Kobe University
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- TANAKA Hiromitsu
- Department of Physiology, School of Medicine, Teikyo University
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- SHIRAKAWA Ibuki
- Department of Physiology, School of Medicine, Teikyo University
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- KARR Trudy
- Department of Biology, Johns Hopkins University
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- SUGI Haruo
- Department of Physiology, School of Medicine, Teikyo University
書誌事項
- タイトル別名
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- Evidence for the Essential Role of Myos
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The role of myosin subfragment-2 (myosin S-2) in muscle contraction was studied by using an in vitro motility assay system in which the ATP-dependent sliding between myosin-coated polystyrene beads and actin filament arrays (actin cables) of giant algal cells were recorded under constant external loads provided with a centrifuge microscope. With antibody to myosin S-2 below 0.3 mg/ml, the maximum “isometric” force generated by myosin molecules on the bead decreased markedly, but the unloaded bead-sliding velocity along actin cables did not change appreciably, indicating a decrease in the number of myosin molecules interacting with actin scables. The antibody at 0.3-1.5 mg/ml decreased not only the maximum isometric force, but also the unloaded bead-sliding velocity in a dose-dependent manner. With the antibody at 1.5-3 mg/ml, the beads eventually stopped moving to remain attached to actin cables. These beads could be readily detached from actin cables with very small centrifugal forces, indicating very weak actin-myosin linkages. The antibody had no effect on rigor actin myosin linkages fromed before the antibody application. These results are consistent with the view that myosin S-2 plays an essential role in muscle contraction.
収録刊行物
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- The Japanese Journal of Physiology
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The Japanese Journal of Physiology 48 (5), 383-387, 1998
一般社団法人 日本生理学会
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詳細情報 詳細情報について
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- CRID
- 1390001205042957056
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- NII論文ID
- 10008305472
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- NII書誌ID
- AA00691224
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- COI
- 1:CAS:528:DyaK1MXlvFWg
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- ISSN
- 18811396
- 0021521X
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- NDL書誌ID
- 4615131
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- PubMed
- 9852347
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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