Biochemical Analysis of Lipopolysaccharides from Respiratory Pathogenic <I>Branhamella catarrhalis</I> Strains and the Role of Anti-LPS Antibodies in <I>Branhamella</I> Respiratory Infections

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  • TANAKA Hiroshi
    Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University
  • OISHI Kazunori
    Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University
  • SONODA Fuminari
    Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University
  • IWAGAKI Akitaka
    Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University
  • NAGATAKE Tsuyoshi
    Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University
  • MATSUMOTO Keizo
    Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University

Bibliographic Information

Other Title
  • 呼吸器病原性ブランハメラ・カタラーリスLPSの生化学的分析と呼吸器感染症患者血清中抗LPS抗体の役割
  • コキュウキ ビョウゲンセイ ブランハメラ カタラーリス LPS ノ セイカガク
  • Biochemical Analysis of Lipopolysaccharides from Respiratory Pathogenic Branhamella catarrhalis Strains and the Role of Anti-LPS Antibodies in Branhamella Respiratory Infections

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Abstract

We characterized lipopolysaccharides (LPSs) from respiratory pathogenic Branhamella catarrhalis (BC) strains, and evaluated the protective property of and-BC LPS antibody in BC respiratory infections. LPSs from four strains of BC were lipooligosaccharide having no 0-side chain and a Mr of 3 KDa, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). All of them produced different patterns, showing two to four bands on SDS-PAGE. We found high level of anti-BC IgG antibody in convalescent sera from a patient with BC respiratory tract infection by ELISA. This IgG antibody recognized BC LPSs on Western blots. Two respiratory pathogens of BC (strains; 87-122, 88-23) were tested in a bactericidal assay employing a convalescent sera. 87-122 strain was susceptible to antibody-dependent, complement-mediated killing, while 88-23 strain was resistant. The killing of 87-122 strain was inhibited by addition of the homologous BC LPS to the convalescent sera in a dose-dependent manner. These data support that anti-BC LPS antibody maymediate complement-lysis of some strains of BC, and play a protective role in BC respiratory infections.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 66 (6), 709-715, 1992

    The Japanese Association for Infectious Diseases

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