A Novel Approach to the Detection of <I>Neisseria gonorrhoeae</I> Employing DNA Probe Method

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  • HARADA Yusuke
    Department of Microbiology, Toho University School of Medicine
  • MIYAZAKI Shuichi
    Department of Microbiology, Toho University School of Medicine
  • KANEKO Yasuko
    Department of Microbiology, Toho University School of Medicine
  • GOTO Sachiko
    Department of Microbiology, Toho University School of Medicine

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Other Title
  • DNAプローブ法による淋菌検出に関する基礎的検討
  • DNA プローブホウ ニ ヨル リンキン ケンシュツ ニ カンスル キソテキ
  • A Novel Approach to the Detection of Neisseria gonorrhoeae Employing DNA Probe Method

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Description

The present study was undertaken to establish a novel detection method for Neisseria gonorrhoeae (N. gonorrhoeae). DNA probe obtained from Gen-probe Inc. was employed and clinically isolated strains (18 species, 81 strains) were applied to verify the quality and quantity of the method.<BR>The present data revealed that the method is specific to detect N. gonorrhoeae (30/30): neither gram positive nor negative oraganisms were detectable by the method (0/51). It has been shown, further more, that there exists a significant correlation between the number of the bacteria and the relative light units (r=0.96). The threshold of cell numbers, on the other hand, was found to be 5 × 103 cfu/tube.<BR>The detection sensitivity for N. gonorrhoeae was not influenced by the presence of other bacteria, and not decreased by using heat-treated or drug-treated N. gonorrhoeae organisms. The present study also provides some experimental evidences that the method can also be applicable for the detection of bacteriolytic action of drugs.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 65 (7), 844-850, 1991

    The Japanese Association for Infectious Diseases

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