乙euconostoc mesenteroides ATCC 12291株由来シュクロースホスホリラーゼの精製と性質および本酵素を用いた二糖の合成
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- 北岡 久美子
- Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd.
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- 高橋 英樹
- Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd.
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- 原 耕三
- Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd.
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- 橋本 仁
- Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd.
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- 佐々木 堯
- National Food Research Institute
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- 谷口 肇
- National Food Research Institute
書誌事項
- タイトル別名
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- Purification and Characterization of Sucrose Phosphorylase from Leuconostoc mesenteroides ATCC 12291 Cells, and Disaccharides Synthesis by the Enzyme
説明
A sucrose phosphorylase was purified from Leuconostoc mesenteroides ATCC 12291 cells to an electrophoretically homogeneous state with hydrophobic, ion-exchange and gel filtration column chromatographies. The enzyme had a molecular weight of 55, 000 and pI of 4.0. It showed the maximum activity at pH 6.5 in a transfer reaction of glucose residue from a-D-glucose -l-phosphate (G-1-P), at pH 7.0 in a transfer reaction from sucrose and is fully stable at pH between 5.5 and 7.5. It showed the maximum activity at 35°C and is stable below 40°C. D-Fructose, D-xylulose, L-arabinose, Lsorbose and L-ribulose can serve as an acceptor in the transfer reaction from G-1-P, and the last four can serve as an acceptor in the reaction from sucrose, too. It was found that the reactions with sucrose gave a higher yield than those with G-1-P . Among the reactions tested, glucosylxyluloside synthesis from sucrose and xylulose gave the highest yield.
収録刊行物
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- 応用糖質科学
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応用糖質科学 41 (2), 165-172, 1994
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001205171445504
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- NII論文ID
- 130004091049
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- COI
- 1:CAS:528:DyaK2MXjtlGnsQ%3D%3D
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- ISSN
- 18844898
- 13403494
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可